EFFECTIVENESS OF BETEL LEAF EXTRACT (Piper betle L) AS AN ANTIBACTERIAL AGENT FOR TREATING BACTERIAL INFECTIONS (Aeromonas hydrophila) IN KOI FISH BREEDS
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Aslamiah*
Maya Istyadji
Ratna Yulinda
Betel leaf has a long history of use in traditional medicine due to its natural antibacterial properties. This study focuses on the antibacterial effects of betel leaf (Piper betle) extract, particularly against Aeromonas hydrophila infections in koi fish. The research employs an experimental approach using a Completely Randomized Design (CRD) with four treatment groups, each repeated twice. The treatments consist of: a control group (Treatment K) without betel leaf extract, Treatment A with 5 ml of betel leaf extract, Treatment B with 10 ml of betel leaf extract, and Treatment C with 15 ml of betel leaf extract. The results of the study demonstrate the significant efficacy of betel leaf extract in promoting the healing of Aeromonas hydrophila-infected fish. This is evident from observations of reduced wound diameter in fish treated with betel leaf extract. In Treatment A (5 ml dose), noticeable wound diameter reduction begins in the fourth week. Treatment B (10 ml dose) shows similar progress with visible healing in the third week. Treatment C (15 ml dose) exhibits the most promising results, with wound diameter reduction or fading evident as early as the second week. Survival rates are recorded as follows: Treatment K – 50%, Treatment A – 65%, Treatment B – 75%, and Treatment C – 90%. These findings underscore the potential of betel leaf extract as an effective antibacterial remedy in combating Aeromonas hydrophila infections in koi fish.
Aznury, M., Hajar, L., & Serlina, A. (2021). Optimasi Formula Pembuatan Sabun Padat Antiseptik Alami dengan Penambahan Ekstrak Daun Sirih Hijau (Piper betle L). Jurnal Kinetika, 12(1), 51-59.
Candrasari, A., Romas, M. A., & Astuti, O. R. (2011). Uji daya antimikroba ekstrak etanol daun sirih merah (Piper crocatum Ruiz & Pav) terhadap Pertumbuhan Staphylococcus aureus, eschericia albicans secara in vitro. Jurnal Biomedika, 4(1), 9-16.
Cholik, F., Artati, & Arifudin, R. (2005). Pengelolaan Kualitas Kolam. INFIS Manual seri nomor 26. Dirjen Perikanan. Jakarta.
Djuma, A. W. (2019). Aktivitas Antimikroba Ekstrak Daun Sirih Hijau (Piper betle L) terhadap Pertumbuhan Bakteri Staphylococcus aureus. Prosiding Semnas Sanitasi, 136-142.
Dwianggraini, R., Pujiastuti, P., & Ermawati, T. (2013). Perbedaan Efektivitas Antibakteri antara Ekstrak Daun Sirih Merah (Piper Crocatum) dan Ekstrak Daun Sirih Hijau (Piper betle L) terhadap Porphyromonas gingivalis. Stomatognatic Jurnal Kedokteran Gigi, 10(1), 1-5.
Kursia, S., Lebang, J. S., & Nursamsiar, N. (2016). Uji Aktivitas Antibakteri Ekstrak Etilasetat Daun Sirih Hijau (Piper Betle L) terhadap Bakteri Staphyloccus epidermis. Indonesia Journal of Pharmaceutic Sciences and Technology, 3(2), 72-77.
Marsono, O. S., Susilorini, T. E., & Surjowardojo, P. (2017). Pengaruh Lama Penyimpanan Dekok Daun Sirih Hijau (Piper Betle L) Terhadap Aktivitas Daya Hambat Bakteri Streptococcus Agalatiae Penyebab Mastitis Pada Sapi Perah. Jurnal Ilmu dan Teknologi Hasil Ternak, 12(1), 47-60.
Patil, R. S., Harale, P. M., Shivangekar, K. V., Khumbhar, P. P., & Desai, R. R. (2015). Phytochemical potential and in vitro antimicrobial activity of Piper betle Linn. Leaf extracts. Journal of Chemical and Pharmaceutical Research, 7(5), 1095-1101.
Sari, D. O., Hadibrata, E., & Oktafany. (2019). Daun Sirih Hijau (Piper betle L) sebagai Pengganti Antibiotik pada Prostatitis. Jurnal Medula, 9(2), 262-266.
Zaeroni, A., Setyowati, D. N., & Fariq, A. (2022). Effect of Betel Leaf Extract (Piper betle L) on Aeromonas hydrophila Diseases in Tilapia (Oreochromis niloticus) Seed. Journal of Fish Health, 2, 66-67.
Zainuddin, Rahmaningsih, S., & Firmani, U. (2018). Pemanfaatan Serbuk Daun Sirih (Piper betle L) untuk Meningkatkan Kesehatan Ikan Nila (Oreochromis nilaticus). Jurnal Perikanan Pantura, 1(1), 16-22.