Evaluation of Embryo Transfer Pregnancy in Beef Cattle
Main Article Content
Elrois Ngongo*
Mohamad Nurul
Akhadiyah Afrila
Embryo transfer is one of the reproductive biotechnologies that enables rapid and controlled improvement in genetic quality and livestock populations. This technology allows embryos resulting from fertilisation in superior donor cows to be implanted into recipient cows, thereby producing offspring with high genetic potential without reducing the reproductive capacity of the donor cows. This study aims to evaluate the success of embryo transfer (ET) in beef cattle and the factors influencing the birth rate of calves resulting from ET in Lamongan District. Embryo transfer is a promising assisted reproductive technology for accelerating genetic improvement in beef cattle. The research method used in this study is a literature review employing a quantitative descriptive approach. The study was conducted on community-owned embryo transfer farms in Lamongan District over two months, using eight beef cattle as recipients. Data collection methods involved obtaining data from secondary sources related to embryo transfer outcomes at community-owned embryo transfer farms in Lamongan District. The variable in the study was pregnancy rate. The results showed that the pregnancy rate reached 50%, consistent with the general success range for ET (40–70%). All pregnancies were achieved through embryo placement in the upper third of the uterine horn and natural oestrus synchronisation. However, all pregnancies ended in abortion during the second trimester (4–6 months of age). The main identified causes include unverified embryo quality, genetic incompatibility, environmental stress, micronutrient deficiencies, and inadequate post-transfer management. The evaluation showed farmers had varying education and experience, but lacked sufficient technical training.
Adriani, A., Sudono, A., Sutardi, T., Manalu, A., & Sutama, I. K. (2007). Prenatal growth in uterus of does by superovulation. HAYATI Journal of Biosciences, 14(2), 44.
Alam, I. (2024). Perbandingan Hasil Program Superovulasi Secara Subkutan pada Bangsa Simental dan Limousin di Balai Embrio Ternak Cipelang Bogor [IPB University]. http://repository.ipb.ac.id/handle/123456789/153862
Baruselli, P. S., Ferreira, R. M., Sá Filho, M. F., Nasser, L. F. T., Rodrigues, C. A., & Bó, G. A. (2009). Bovine embryo transfer recipient synchronisation and management in tropical environments. Reproduction, Fertility and Development, 22(1), 67–74.
Bilyaro, W., Rafian, T., Azis, A. R., & Dani, M. (2024). Upaya Meningkatkan Produksi Sapi Potong melalui Penerapan Genetika. Journal of Agriculture and Animal Science, 4(2), 84–89.
Bó, G. A., Cedeño, A., & Mapletoft, R. J. (2019). Strategies to increment in vivo and in vitro embryo production and transfer in cattle. Animal Reproduction, 16, 411–422.
Diskin, M. G., & Morris, D. G. (2008). Embryonic and early foetal losses in cattle and other ruminants. Reproduction in Domestic Animals, 43, 260–267.
Gebremedhn, S., Salilew-Wondim, D., Hoelker, M., Rings, F., Neuhoff, C., Tholen, E., Schellander, K., & Tesfaye, D. (2016). MicroRNA-183-96-182 cluster regulates bovine granulosa cell proliferation and cell cycle transition by coordinately targeting FOXO1. Biology of Reproduction, 94(6), 121–127.
Hansen, P. J. (2020). The incompletely fulfilled promise of embryo transfer in cattle—why aren’t pregnancy rates greater and what can we do about it? Journal of Animal Science, 98(11), skaa288.
Hapari, A. (2022). Tingkat keberhasilan transfer embrio (te) berdasarkan kegiatan seleksi resipien dan pelaksanaan te dari tahun 2018 sampai dengan 2021 di kabupaten Rokan Hulu. SUNGKAI, 10(2), 38–52.
Hasler, J. F. (2001). Factors affecting frozen and fresh embryo transfer pregnancy rates in cattle. Theriogenology, 56(9), 1401–1415.
Hasler, J. F. (2014). Forty years of embryo transfer in cattle: A review focusing on the journal Theriogenology, the growth of the industry in North America, and personal reminisces. Theriogenology, 81(1), 152–169.
Ihsan, M. N., & Wahjuningsih, S. (2011). Penampilan reproduksi sapi potong di Kabupaten Bojonegoro. TERNAK TROPIKA Journal of Tropical Animal Production, 12(2), 74–77.
Kadarmideen, H. N., Mazzoni, G., Watanabe, Y. F., Strøbech, L., Baruselli, P. S., Meirelles, F. V., Callesen, H., Hyttel, P., Ferraz, J. B. S., & Nogueira, M. F. G. (2018). Genomic selection of in vitro produced and somatic cell nuclear transfer embryos for rapid genetic improvement in cattle production. Animal Reproduction (AR), 12(3), 389–396.
Lonergan, P., & Fair, T. (2016). Maturation of oocytes in vitro. Annual Review of Animal Biosciences, 4(1), 255–268.
Lonergan, P., Fair, T., Forde, N., & Rizos, D. (2016). Embryo development in dairy cattle. Theriogenology, 86(1), 270–277.
Maillo, V., Lopera-Vasquez, R., Hamdi, M., Gutierrez-Adan, A., Lonergan, P., & Rizos, D. (2016). Maternal-embryo interaction in the bovine oviduct: Evidence from in vivo and in vitro studies. Theriogenology, 86(1), 443–450.
Mikkola, M. (2017). Superovulation and embryo transfer in dairy cattle: effect of management factors with emphasis on sex-sorted semen.
Nurkhasanah, Y. (2016). Tingkat Keberhasilan Transfer Embrio dari Donor Sapi Simmental dan Limousin di BET Cipelang Bogor.
Otoluwa, M. A., Salendu, A. H. S., Rintjap, A. K., & Massie, M. T. (2016). Prospek Pengembangan Usaha Ternak Sapi Potong Di Kecamatan Bolangitang Timur Kabupaten Bolaang Mongondow Utara. Zootec, 36(1), 191–197.
Rizal, D. M. (2003). Kualitas Embrio, Interferon Gamma, Waktu Transfer Dan Kegagalan Implantasi Embrio. Program Pendidikan Pasca sarjana Universitas Diponegoro.
Roth, Z. (2018). Stress‐induced alterations in oocyte transcripts are further expressed in the developing blastocyst. Molecular Reproduction and Development, 85(11), 821–835.
Siqueira, L. G., Tribulo, P., Chen, Z., Denicol, A. C., Ortega, M. S., Negrón-Pérez, V. M., Kannampuzha-Francis, J., Pohler, K. G., Rivera, R. M., & Hansen, P. J. (2017). Colony-stimulating factor 2 acts from days 5 to 7 of development to modify programming of the bovine conceptus at day 86 of gestation. Biology of Reproduction, 96(4), 743–757.
Situmorang, P., & Triwulaningsih, E. (2004). Aplikasi dan inovasi teknologi transfer embrio (TE) untuk pengembangan sapi potong. Lokakarya Nasional Sapi Potong, 95–105.
Spell, A. R., Beal, W. E., Corah, L. R., & Lamb, G. C. (2001). Evaluating recipient and embryo factors that affect pregnancy rates of embryo transfer in beef cattle. Theriogenology, 56(2), 287–297.
Vasconcelos, J. L. M., Carvalho, R., Peres, R. F. G., Rodrigues, A. D. P., Junior, I. C., Meneghetti, M., Aono, F. H., Costa, W. M., Lopes, C. N., & Cooke, R. F. (2018). Reproductive programs for beef cattle: incorporating management and reproductive techniques for better fertility. Animal Reproduction (AR), 14(3), 547–557.
Velazquez, M. A. (2008). Assisted reproductive technologies in cattle: applications in livestock production, biomedical research and conservation biology. Annual Review of Biomedical Sciences, 10.









