Potential Effectiveness of Secondary Metabolites of Azolla microphylla as Aedes aegypti Repellent Guide
Main Article Content
Muhammad Sungging Pradana*
Evy Ratnasari Ekawati
Siti Nur Husnul Yusmiati
Imron Rosady
Ridanti Nagita Cahyani
Dengue Hemorrhagic Fever (DHF) remains an endemic public health concern, with Aedes aegypti mosquitoes serving as the primary transmission vector. While chemical repellents are widely used for vector control, their health and environmental risks have driven the need for safer, biodegradable natural alternatives. This study aimed to identify the secondary metabolite content of Azolla microphylla leaf extract and evaluate its effectiveness as a repellent against Aedes aegypti. This experimental study employed a post-test only control group design. Three extract concentrations (10%, 15%, and 20%) were tested alongside positive and negative controls, using 10 mosquitoes per group. Observations were conducted at 1, 2, 4, and 6 hours, with data analyzed using statistical tests to assess differences between treatment groups. Phytochemical screening confirmed the presence of flavonoids, terpenoids, and tannins in the extract. Repellent testing revealed that the 20% concentration provided the highest effectiveness, with an average mosquito repellency of 58% during the first 1–2 hours. However, effectiveness declined progressively across all concentrations over time. Statistical analysis confirmed a significant difference between treatment and control groups after 1 hour of observation (p < 0.05). Azolla microphylla leaf extract demonstrates potential as a natural repellent against Aedes aegypti; however, its protective effect is temporary due to the volatility of its active compounds. Further formulation development, such as encapsulation or the use of fixative agents, is needed to enhance the stability and durability of its repellent activity.
Adnani, B., Rahmah, Z., Fitrianingsih, A. A., & Setiawan, A. M. (2020). Potential Test Of Ethanol Extract From Onion (Allium Cepa L) Leaves As A Repellent To Aedes Aegypti. Journal of Islamic Medicine, 4(2), 65–75. https://doi.org/10.18860/jim.v4i2.10171
Adnyana, I. M. D. M., Sudaryati, N. L. G., & Sitepu, I. (2021). Toxicity of Legiayu incense as Insecticide and Larvicide Against Aedes aegypti Mosquitoes Mortality. Indonesian Journal of Pharmacy, 32(4), 524–521. https://doi.org/10.22146/ijp.1814
Akbar, N. H., Manurung, I. F. E., & Regaletha, T. A. L. (2022). The Effectiveness of Moringa (Moringa Oleifera) Bark Extract As A Repellent Against Aedes Aegypti. Pancasakti Journal Of Public Health Science And Research, 2(2), 75–80. https://doi.org/10.47650/pjphsr.v2i2.395
Bayuadi, Purwati, & Panjaitan, R. S. (2023). Repellent Activity Test of Essential Oil Gel of Cinnamommum Burmanii Bi Leaves Against Aedes Aegypti Mosquitoes. Indonesian Journal of Pharmaceutical Research, 2(2), 34–44. https://doi.org/10.31869/ijpr.v2i2.4180
Chanapanchai, S., Fitriya, W., Bagus, I., Artadana, M., & Supaibulwatana, K. (2025). Important role and benefits of Azolla plants in the management of agroecosystem services , biodiversity , and sustainable rice production in Southeast Asia. Journal of Integrative Agriculture, 24(8), 3004–3023. https://doi.org/10.1016/j.jia.2025.02.027
Dinas Kesehatan Provinsi Jawa Timur. (2024). Profil kesehatan provinsi jawa timur tahun 2023. Dinas Kesehatan Provinsi Jawa Timur. https://www.scribd.com/document/765987033/Profil-Kesehatan-Provinsi-Jawa-Timur-Tahun-2023
Ekawati, E. R., Darmanto, W., & Wahyuningsih, S. P. A. (2021). Evaluation of the Bioactivity of MeOH:DMSO (1:1, v/v) Lime Peel Extract on Methicillin-Resistant Staphylococcus aureus. Journal of Hunan University (Natural Sciences), 48(5). https://jonuns.com/index.php/journal/article/view/568
Ekawati, E. R., & Pradana, M. S. (2019). The Effectiveness of Azolla pinnata in Inhibiting the Growth of Salmonella typhi. Jurnal Biota, 5(1), 1–5. https://doi.org/10.19109/biota.v5i1.2696
Felipe Oliveros-Díaz, A., Pájaro-González, Y., Cabrera-Barraza, J., Hill, C., Quiñones-Fletcher, W., Olivero-Verbel, J., & Díaz Castillo, F. (2022). Larvicidal activity of plant extracts from Colombian North Coast against Aedes aegypti L. mosquito larvae. Arabian Journal of Chemistry, 15(12), 104365. https://doi.org/10.1016/j.arabjc.2022.104365
Irfayanti, N. A., Jasmiadi, & Tari, A. (2022). Formulation and Activity Test of Repellent Spray Marigold Flower Essential Oil (Tagetes erecta L.) in Aedes aegypti Mosquitoes. Journal Syifa Sciences and Clinical Research, 4(2), 363–370. https://doi.org/10.37311/jsscr.v4i2.14161
Kaur, S., Kumar, M., Sharma, P., Sharma, I., Upadhyay, S. K., & Singh, R. (2022). Preparation and applications of anti-mosquito herbal dhoopwati by using mosquito repellent plants. Bulletin of Pure & Applied Sciences- Botany, 41b(1), 66–70. https://doi.org/10.5958/2320-3196.2022.00006.4
Kemenkes. (2024). Profil Kesehatan Indonesia 2024. Kementerian Kesehatan Republik Indonesia. https://kemkes.go.id/id/profil-kesehatan-indonesia-2024
Kraemer, M. U., Sinka, M. E., Duda, K. A., Mylne, A. Q., Shearer, F. M., Barker, C. M., Moore, C. G., Carvalho, R. G., Coelho, G. E., Van Bortel, W., Hendrickx, G., Schaffner, F., Elyazar, I. R., Teng, H.-J., Brady, O. J., Messina, J. P., Pigott, D. M., Scott, T. W., Smith, D. L., … Hay, S. I. (2015). The global distribution of the arbovirus vectors Aedes aegypti and Ae. albopictus. ELife, 4, e08347. https://doi.org/10.7554/eLife.08347
Larasati, D., & Putri, F. M. S. (2023). Skrining Fitokimia dan Penentuan Kadar Flavonoid Ekstrak Etanol Limbah Kulit Pisang (Musa acuminata Colla). Jurnal Mandala Pharmacon Indonesia, 9(1), 125–131. https://doi.org/10.35311/jmpi.v9i1.330
Macdonald, O., Adeboye, O., Ngozi, R., Olatunbosun, S., & Author, C. (2016). Fractionation and Identification of Bioactive Constituents From Sapium Ellipticum (Hochst) Leaf Extract. Animal Research International, 13(3), 2492–2503. https://www.proquest.com/docview/1857759991?pq-origsite=gscholar&fromopenview=true&sourcetype=Scholarly Journals
Maia, M. F., & Moore, S. J. (2011). Plant-based insect repellents: a review of their efficacy, development and testing. Malaria Journal, 10(S1). https://doi.org/10.1186/1475-2875-10-s1-s11
Meier, C. J., Rouhier, M. F., & Hillyer, J. F. (2022). Chemical Control of Mosquitoes and the Pesticide Treadmill: A Case for Photosensitive Insecticides as Larvicides. Insects, 13(12), 1093. https://doi.org/10.3390/insects13121093
Messina, J. P., Brady, O. J., Golding, N., Kraemer, M. U. G., Wint, G. R. W., Ray, S. E., Pigott, D. M., Shearer, F. M., Johnson, K., & Earl, L. (2019). The current and future global distribution and population at risk of dengue. Nature Microbiology, 4(9), 1508–1515. https://doi.org/10.1038/s41564-019-0476-8
Mirmanto, Syahrul, Sulistyowati, E. D., Okariawan, I. D. K., & Rodian. (2017). Effect of inlet temperature and ventilation on heat transfer rate and water content removal of red chilies. Journal of Mechanical Science and Technology, 31(3), 1531–1537. https://doi.org/10.1007/s12206-017-0252-8
Mukabana, W. R., Mweresa, C. K., Otieno, B., Omusula, P., Smallegange, R. C., van Loon, J. J. A., & Takken, W. (2012). A novel synthetic odorant blend for trapping of malaria and other African mosquito species. Journal of Chemical Ecology, 38(3), 235–244. https://doi.org/10.1007/s10886-012-0088-8
Nogata, Y., Sakamoto, K., Shiratsuchi, H., Ishii, T., Yano, M., & Ohta, H. (2006). Flavonoid composition of fruit tissues of citrus species. Bioscience, Biotechnology and Biochemistry, 70(1), 178–192. https://doi.org/10.1271/bbb.70.178
Nuchuchua, O., Sakulku, U., Uawongyart, N., Puttipipatkhachorn, S., Soottitantawat, A., & Ruktanonchai, U. (2009). In vitro characterization and mosquito (Aedes aegypti) repellent activity of essential-oils-loaded nanoemulsions. Aaps Pharmscitech, 10(4), 1234–1242. https://doi.org/10.1208/s12249-009-9323-1
Obembe, A., & Oso, O. G. (2022). Larvicidal Effects of Citrus Peels Extracts against Culex Pipiens Mosquitoes. Althea Medical Journal, 9(4), 185–190. https://doi.org/10.15850/amj.v9n4.2786
Patel, E. K., Gupta, A., & Oswal, R. J. (2012). A Review on: Mosquito Repellent Methods. International Journal of Pharmaceutical, Chemical and Biological Sciences, 2(3), 310–317. https://www.ijpcbs.com/articles/a-review-on-mosquito-repellent-methods.pdf
Patni, W. S. D., Wahyuni, A., Jausal, A. N., & Busman, H. (2024). Literature Review : Hubungan Antara Jenis Kelamin dan Jenis Operasi Terhadap Kejadian Post Operative Nausea Vomitting. Jurnal Kesehatan Dan Agromedicine, 11(2), 32–39. https://doi.org/10.23960/jka.v11i2.pp32-39
Prasetyo, B. F. (2021). Uji Aktivitas Antioksidan dan Daya Hambat Enzim Tirosinase Ekstrak Etanol Azolla filiculoides Lam. Jurnal Sains Farmasi & Klinis, 8(1), 53. https://doi.org/10.25077/jsfk.8.1.53-59.2021
Qian, Y., Han, Q.-H., Wang, L.-C., Guo, Q., Wang, X.-D., Tu, P.-F., Zeng, K.-W., & Liang, H. (2018). Total saponins of Albiziae Cortex show anti-hepatoma carcinoma effects by inducing S phase arrest and mitochondrial apoptosis pathway activation. Journal of Ethnopharmacology, 221, 20–29. https://doi.org/10.1016/j.jep.2018.04.015
Rashad, S. (2021). An overview on the aquatic fern Azolla spp. as a sustainable source of nutrients and bioactive compounds with resourceful applications Sayed Rashad. Egyptian Journal of Aquatic Biology & Fisheries, 25(1), 775–782. https://doi.org/10.21608/ejabf.2021.150205
Ravi, R., Rajendran, D., Oh, W. Da, Mat Rasat, M. S., Hamzah, Z., Ishak, I. H., & Mohd Amin, M. F. (2020). The potential use of Azolla pinnata as an alternative bio-insecticide. Scientific Reports, 10(1), 1–9. https://doi.org/10.1038/s41598-020-75054-0
Ravi, R., Zulkrnin, N. S. H., Rozhan, N. N., Yusoff, N. R. N., Rasat, M. S. M., Ahmad, M. I., Ishak, I. H., & Amin, M. F. M. (2018). Chemical composition and larvicidal activities of Azolla pinnata extracts against Aedes (Diptera:Culicidae). PLoS ONE, 13(11), 1–18. https://doi.org/10.1371/journal.pone.0206982
Rohmah, E. A., Subekti, S., & Rudyanto, M. (2020). Larvicidal Activity and Histopathological Effect of Averrhoa bilimbi Fruit Extract on Aedes aegypti from Surabaya, Indonesia. Journal of Parasitology Research, 2020, 1–5. https://doi.org/10.1155/2020/8866373
Sarni, Anwar, R., & Sayono. (2023). Aktivitas Repelensi Ekstrak Etil Asetat dan Metanol Rimpang Lengkuas Terhadap Nyamuk Aedes aegypti dan Aedes albopictus. Prosiding Seminar Kesehatan Masyarakat, 1(Oktober), 11–18. https://doi.org/10.26714/pskm.v1ioktober.233
Sharma, S., Verma, D. A., & Srivastava, N. (2024). A review on medicinal plants having mosquito repellents activity. Journal of Pharmacognosy and Phytochemistry, 13(3), 82–85. https://doi.org/10.22271/phyto.2024.v13.i3b.14944
Shenashen, M., Derbalah, A., Hamza, A., Mohamed, A., & El Safty, S. (2017). Antifungal activity of fabricated mesoporous alumina nanoparticles against root rot disease of tomato caused by Fusarium oxysporium. Pest Management Science, 73(6), 1121–1126. https://doi.org/10.1002/ps.4420
Smallegange, R. C., Qiu, Y. T., van Loon, J. J. A., & Takken, W. (2005). Synergism between ammonia, lactic acid and carboxylic acids as kairomones in the host-seeking behaviour of the malaria mosquito Anopheles gambiae sensu stricto (Diptera: Culicidae). Chemical Senses, 30(2), 145–152. https://doi.org/10.1093/chemse/bji010
Specos, M. M. M., García, J. J., Tornesello, J., Marino, P., Vecchia, M. Della, Tesoriero, M. V. D., & Hermida, L. G. (2010). Microencapsulated citronella oil for mosquito repellent finishing of cotton textiles. Transactions of the Royal Society of Tropical Medicine and Hygiene, 104(10), 653–658. https://doi.org/10.1016/j.trstmh.2010.06.004
Suari, L. G. S. A., Haq, A. D., & Rahayu, L. A. D. (2021). Potensi Ekstrak Bunga Kamboja (Plumeria sp.) dan Bunga Kluwih (Artocarpus camansi) sebagai Biolarvasida Nyamuk Anopheles sp. dalam Upaya Pencegahan Penyakit Malaria. JIMKI: Jurnal Ilmiah Mahasiswa Kedokteran Indonesia, 8(3), 137–145. https://doi.org/10.53366/jimki.v8i3.267
Terradas, G., Manzano-Alvarez, J., Vanalli, C., Werling, K., Cattadori, I. M., & Rasgon, J. L. (2024). Temperature affects viral kinetics and vectorial capacity of Aedes aegypti mosquitoes co-infected with Mayaro and Dengue viruses. Parasites & Vectors, 17(1), 73. https://doi.org/10.1186/s13071-023-06109-0
Thomas, S. J. (2023). Is new dengue vaccine efficacy data a relief or cause for concern? Npj Vaccines, 8(1), 55. https://doi.org/10.1038/s41541-023-00658-2
Widiastara, A. A., Mudjianto, G. P., Rohmah, E. A., Putra, H. A., Ningtyas, M. I. W., Sulistyawati, S. W., Fitriah, S. P., Indriyani, K. D., Athiyyah, A. F., & Basuki, S. (2022). The Longevity of Aedes aegypti Larvae in Several Water Sources in Surabaya. Indonesian Journal of Tropical and Infectious Disease, 10(1), 18–26. https://doi.org/10.20473/ijtid.v10i1.32209
Widiyaningtiyas, M. I., Putra, R. A., Ekawati, E. R., Herawati, D., & Mustika, A. (2025). The Effect of a Combination of Ethanol Extract from Leaves and Flowers of Plumeria acuminate L . Against Aedes aegypti Larvae. Journal of Multidisciplinary Applied Natural Science, 5(1), 18–31. https://doi.org/10.47352/jmans.2774-3047.225
Wilke, A. B. B., Vasquez, C., Carvajal, A., Ramirez, M., Cardenas, G., Petrie, W. D., & Beier, J. C. (2021). Effectiveness of adulticide and larvicide in controlling high densities of Aedes aegypti in urban environments. Journal Plos One, 16(1), 1–15. https://doi.org/10.1371/journal.pone.0246046
Wilson, J. J., Mahendran, S., Sivakumar, T., Ponmanickam, P., & Thangaraj, R. (2023). Mosquito larvicidal activity of silver nanoparticles synthesized using Azolla pinnata against Culex quinquefasciatus Say (Diptera: Culicidae). South African Journal of Botany, 157, 380–386. https://doi.org/10.1016/j.sajb.2023.04.019
Yu, F., Amer, S., Qi, M., Wang, R., Wang, Y., Zhang, S., Jian, F., Ning, C., El Batae, H., & Zhang, L. (2019). Multilocus genotyping of Giardia duodenalis isolated from patients in Egypt. Acta Tropica, 196, 66–71. https://doi.org/10.1016/j.actatropica.2019.05.012
