Histological and Biochemical Effect of Branded Energy Drink (Fearless) on the Liver and Kidney of Albino Rats
Goyang J. Tsennoe
Gongmen T. Tokwap
Solomon M. Gamde*
Imoh Ibanga
James O. Adisa
Energy drink consumption is rising globally due to their perceived mental and physical performance benefits. These carbonated beverages contain high levels of caffeine, sugar, and various additives. This study examined the effects of the energy drink (Fearless) on liver and kidney histology and biochemical parameters in albino rats. Fifteen Rattus norvegicus (mean weight 126.3±23.9g) were divided into three groups (n=5): Group A (control) received standard feed and water; Groups B and C received 10 ml/kg and 20 ml/kg body weight/day of the energy drink for 6 weeks, respectively. The body weights of animals were measured. Following sacrifice under light anesthesia, blood samples were collected for biochemical analysis, and liver and kidney tissues were processed via paraffin wax for histological examination. Body weights of animals descriptively indicated steady increased. Biochemical analysis showed no statistically significant changes in the kidney function serum sodium, potassium, chloride, urea, and creatinine inclusive compared to controls. However, liver function parameters showed statistically significant elevations (P<0.05) in AST, ALT, and ALP in the test groups. Liver histology revealed central vein congestion and hepatocyte necrosis in both dose groups. The low-dose kidney sections exhibited glomerular tuft changes, polymorphonuclear inflammatory infiltration, architectural distortion, and vascular congestion, while the high-dose group showed widened Bowman's capsule space and renal tubule necrosis with inflammatory infiltration. These findings suggest that chronic energy drink consumption adversely affects liver and kidney structure and function.
Adjene, J., Emojevwe, V., & Idiapho, D. (2014). Effects of long-term consumption of energy drinks on the body and brain weights of adult Wistar rats. Journal of Experimental and Clinical Anatomy, 13(1), 17–20. https://doi.org/10.4103/1596-2393.142925
Aninweze, C. J., Ogbodo, E. C., Onah, C. E., Onyema-Iloh, O. B., Ogalagu, R. O., Onuora, I. J., Okezie, O. A., Olisah, M. C., Okwara, J. E., & Meludu, S. C. (2025). Impact of Energy Drink Consumption on Plasma Urea, Creatinine, Uric Acid, and Electrolytes Among Students of the College of Health Sciences in Okofia Nnewi, Nigeria. Tropical Journal of Phytochemistry and Pharmaceutical Sciences, 4(7), 314–319. https://doi.org/10.26538/tjpps/v4i7.5
Armstrong, L. E. (2002). Caffeine, Body Fluid-Electrolyte Balance, and Exercise Performance. International Journal of Sport Nutrition and Exercise Metabolism, 12(2), 189–206. https://doi.org/10.1123/ijsnem.12.2.189
Bano, S. S., Ali, S., Rana, R., Ali, H., Ahmad, A., & Khurshid, T. (2020). Histological effects of caffeinated energy drink consumption and its withdrawal on kidneys of experimental rats. Journal of Islamic International Medical College (JIIMC), 15(2), 128–132. https://journals.riphah.edu.pk/index.php/jiimc/article/view/1224
Boone, L., Meyer, D., Cusick, P., Ennulat, D., Bolliger, A. P., Everds, N., Meador, V., Elliott, G., Honor, D., Bounous, D., & Jordan, H. (2005). Selection and interpretation of clinical pathology indicators of hepatic injury in preclinical studies. Veterinary Clinical Pathology, 34(3), 182–188. https://doi.org/10.1111/j.1939-165X.2005.tb00041.x
Buxton, C., & Hagan, J. E. (2012). A survey of energy drinks consumption practices among student -athletes in Ghana: lessons for developing health education intervention programmes. Journal of the International Society of Sports Nutrition, 9(1), 1–8. https://doi.org/10.1186/1550-2783-9-9
Curran, C. P., & Marczinski, C. A. (2017). Taurine, caffeine, and energy drinks: Reviewing the risks to the adolescent brain. Birth Defects Research, 109(20), 1640–1648. https://doi.org/10.1002/bdr2.1177
Gamde, S. M., Ugwah-Oguejiofor, C. J., Garba, A., Avwioro, G. O., Akinpelu, M., & Jimoh, A. A. (2023). Histologic and Biochemical Effect of Balanite aegyptiaca Fruit Extract on Alloxan-Induced Diabetes in Wistar Rats. Ethiopian Journal of Health Sciences, 33(3), 441–450. https://doi.org/10.4314/ejhs.v33i3.7
Gheith, I. (2017). Clinical Pathology of caffeinated and non-caffeinated energy drinks: Review. Life Science Journal, 14(9), 21–36. https://www.lifesciencesite.com/lsj/life140917/03_32766lsj140917_21_36.pdf
Giknis, M. L. A., & Clifford, C. B. (2006). Clinical Laboratory Parameters for Crl:CD(SD) Rats. Charles River Laboratories. https://www.criver.com/resources/rmrmrclinicalparameterscdrat06
Gounden, V., Bhatt, H., & Jialal, I. (2024). Renal function tests. StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK507821/
Greaves, P. (2011). Histopathology of preclinical toxicity studies: interpretation and relevance in drug safety evaluation. Academic Press.
Greene, E., Oman, K., & Lefler, M. (2014). Energy Drink–Induced Acute Kidney Injury. Annals of Pharmacotherapy, 48(10), 1366–1370. https://doi.org/10.1177/1060028014541997
Hall, R. L. (2007). Clinical pathology of laboratory animals. Animal Models in Toxicology, 2, 787–830. https://cir.nii.ac.jp/crid/1573105974752181376
Higgins, J. P., Babu, K., Deuster, P. A., & Shearer, J. (2018). Energy Drinks: A Contemporary Issues Paper. Current Sports Medicine Reports, 17(2), 65–72. https://doi.org/10.1249/JSR.0000000000000454
Kutia, S., Kriventsov, M., Moroz, G., Gafarova, E., & Trofimov, N. (2019). Implications of energy drink consumption for hepatic structural and functional changes: a review. Nutrition & Food Science, 50(5), 937–953. https://doi.org/10.1108/NFS-08-2019-0260
Miller, K. E. (2008). Energy Drinks, Race, and Problem Behaviors Among College Students. Journal of Adolescent Health, 43(5), 490–497. https://doi.org/10.1016/j.jadohealth.2008.03.003
Murt, A. (2025). Energy drink-induced acute kidney injury: a case report and review of the literature. Journal of Medical Case Reports, 19(1), 522. https://doi.org/10.1186/s13256-025-05614-3
Olaleru, F., & Odeigah, P. (2015). Effects of Energy Drink on Sperm Morphology, Haematological Parametres and Behaviour of Adult Male Mice. Annual Research & Review in Biology, 6(5), 288–296. https://doi.org/10.9734/ARRB/2015/13573
Ozer, J., Ratner, M., Shaw, M., Bailey, W., & Schomaker, S. (2008). The current state of serum biomarkers of hepatotoxicity. Toxicology, 245(3), 194–205. https://doi.org/10.1016/j.tox.2007.11.021
Petterino, C., & Argentino-Storino, A. (2006). Clinical chemistry and haematology historical data in control Sprague-Dawley rats from pre-clinical toxicity studies. Experimental and Toxicologic Pathology, 57(3), 213–219. https://doi.org/10.1016/j.etp.2005.10.002
Qassim, A. H., Alsammak, M. A., & Ayoob, A. A. (2022). Histopathological changes in kidney and pancreas induced by energy drinks in adult male rats. Iraqi Journal of Veterinary Sciences, 36(1), 111–116. https://doi.org/10.33899/ijvs.2021.129435.1647
Rahmadi, M., Izzah, Z., Nurhan, A. D., & Suharjono, S. (2024). Chronic intake of energy drinks affects changes in kidney function biomarkers in a diabetes mellitus animal model. Pharmacy Education, 24(3), 25–31. https://doi.org/10.46542/pe.2024.243.2531
Reissig, C. J., Strain, E. C., & Griffiths, R. R. (2009). Caffeinated energy drinks—A growing problem. Drug and Alcohol Dependence, 99(1–3), 1–10. https://doi.org/10.1016/j.drugalcdep.2008.08.001
Seifert, S. M., Schaechter, J. L., Hershorin, E. R., & Lipshultz, S. E. (2011). Health Effects of Energy Drinks on Children, Adolescents, and Young Adults. Pediatrics, 127(3), 511–528. https://doi.org/10.1542/peds.2009-3592
Thapa, B. R., & Walia, A. (2007). Liver function tests and their interpretation. The Indian Journal of Pediatrics, 74(7), 663–671. https://doi.org/10.1007/s12098-007-0118-7
Thoolen, B., Maronpot, R. R., Harada, T., Nyska, A., Rousseaux, C., Nolte, T., Malarkey, D. E., Kaufmann, W., Küttler, K., Deschl, U., Nakae, D., Gregson, R., Vinlove, M. P., Brix, A. E., Singh, B., Belpoggi, F., & Ward, J. M. (2010). Proliferative and Nonproliferative Lesions of the Rat and Mouse Hepatobiliary System. Toxicologic Pathology, 38(7_suppl), 5S-81S. https://doi.org/10.1177/0192623310386499
Uwaifo, G. I. (2019). Beware Energy Drinks: A Case of a Toxic Triad Syndrome in a Diabetic Patient With Nonalcoholic Fatty Liver Disease. The American Journal of the Medical Sciences, 358(4), 304–311. https://doi.org/10.1016/j.amjms.2019.07.015
Valle, M. T. C., Couto-Pereira, N. S., Lampert, C., Arcego, D. M., Toniazzo, A. P., Limberger, R. P., Dallegrave, E., Dalmaz, C., Arbo, M. D., & Leal, M. B. (2018). Energy drinks and their component modulate attention, memory, and antioxidant defences in rats. European Journal of Nutrition, 57(7), 2501–2511. https://doi.org/10.1007/s00394-017-1522-z
Vercammen, K. A., Koma, J. W., & Bleich, S. N. (2019). Trends in Energy Drink Consumption Among U.S. Adolescents and Adults, 2003–2016. American Journal of Preventive Medicine, 56(6), 827–833. https://doi.org/10.1016/j.amepre.2018.12.007
Wee, J. H., Min, C., Park, M. W., Park, I.-S., Park, B., & Choi, H. G. (2021). Energy-drink consumption is associated with asthma, allergic rhinitis, and atopic dermatitis in Korean adolescents. European Journal of Clinical Nutrition, 75(7), 1077–1087. https://doi.org/10.1038/s41430-020-00812-2